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BP207PSemester 21 creditsPractical

Biochemistry (Practical)

Complete unit-wise syllabus for BP207P as per the PCI B.Pharm NEP 2020 curriculum (Semester 2 — Organic Chemistry + Biostatistics).

URL:https://pharmacode.vercel.app/syllabus/semester-2/bp207p-biochemistry-practical/

Course Objectives

  • To develop skills to identify and differentiate carbohydrates and proteins through classical qualitative biochemical tests.
  • To apply biochemical techniques for analysis of pathological conditions using urine and blood samples.
  • To perform estimations of clinically important biomolecules such as glucose, cholesterol, urea, creatinine, uric acid, and proteins in biological fluids.
  • To demonstrate enzymatic activity and study factors affecting enzyme function, including substrate concentration and temperature.
  • To interpret biochemical results and correlate them with clinical conditions such as diabetes, renal dysfunction, and lipid disorders.

Unit-wise Syllabus

1 Units
1
Laboratory Practicals & Experiments (45 Hours)45 Hours
  • 1. Identification tests for proteins (Albumin and Casein).
  • 2. Qualitative analysis of carbohydrates* (Glucose, Fructose, Lactose, Sucrose, and Starch).
  • 3. Qualitative analysis of urine for normal and abnormal constituents.
  • 4. Estimation of blood glucose.
  • 5. Estimation of total cholesterol and HDL cholesterol.
  • 6. Estimation of urea, creatinine, and uric acid in serum.
  • 7. Estimation of serum total protein and albumin.
  • 8. Study of enzymatic hydrolysis of starch.
  • 9. Study of the effect of temperature on salivary amylase activity.
  • 10. Study of the effect of substrate concentration on salivary amylase activity.
  • 11. Estimation of hemoglobin in blood by Sahli’s method / Cyanmethemoglobin method.
  • 12. Estimation of serum bilirubin (total and direct bilirubin).
  • 13. Estimation of glycogen content
  • 14. Estimation of SGOT and SGPT

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Recommended Textbooks & References

  1. Plummer, D. T. An Introduction to Practical Biochemistry. McGraw-Hill, New York.
  2. Wilson, K., and Walker, J. Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press.
  3. Varley, H., Gowenlock, A. H., Bell, M., and Bell, J. L. Varley’s Practical Clinical Biochemistry. Heinemann Medical Books.
  4. Burtis, C. A., Ashwood, E. R., and Bruns, D. E. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Elsevier.
  5. Rodwell, V. W., Bender, D. A., Botham, K. M., Kennelly, P. J., and Weil, P. A. Harper’s Illustrated Biochemistry. McGraw-Hill.
  6. Freifelder, D. Essential Molecular Biology: A Practical Approach. Oxford University Press.

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