BP408PSemester 41 creditsPractical
Pharmaceutical Biotechnology (Practical)
Complete unit-wise syllabus for BP408P as per the PCI B.Pharm NEP 2020 curriculum (Semester 4 — Medicinal Chemistry + 1st Internship).
URL:
https://pharmacode.vercel.app/syllabus/semester-4/bp408p-pharmaceutical-biotechnology-practical/Course Objectives
- Provide fundamental knowledge of biotechnology concepts, including protein therapeutics, monoclonal antibodies, enzyme immobilization, and their pharmaceutical applications.
- Explain the principles and applications of genetic engineering and recombinant DNA technology in pharmaceutical sciences.
- Develop understanding of microbial biotransformation, fermentation processes, and large-scale production of pharmaceutical products such as alcohol, antibiotics, vaccines, and blood products.
- Describe the principles, methodologies, delivery systems, and ethical considerations associated with gene therapy and modern vaccine technologies.
- Introduce the regulatory requirements and standardization procedures for vaccines, sera, and other biopharmaceutical products.
Unit-wise Syllabus
1 Units1
Laboratory Practicals & Experiments (45 Hours)45 Hours- 1. Understanding Good microbiological laboratory practices while working in Microbiology laboratory aseptic handling in microbiology lab: growth and isolation of microbes using streaking, spreading and subculturing techniques: microbial staining, study of various equipments such as loop, straight wire, spreader, forceps, pipette, test tube, petridish, burner etc and apparatus used in microbiology lab- BOD incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer and microscopes.
- 2. Handling of biological spill, decontamination procedures and hygiene while handling microorganisms and disposal
- 3. Sterilization and evaluation of glassware and apparatus using hot air oven
- 4. Preparation and sterilization of solid and liquid culture medium using different techniques
- 5. Determination of microbiological efficacy of disinfectant/ preservative efficacy test
- 6. Tests for sterility of ophthalmic or parenteral marketed formulation according to IP.
- 7. Analysis of Biotechnological product (Protein, nucleic acid materials) by UV Vis and FTIR spectrophotometer
- 8. Production of alcohol using Fermentation process
- 9. Practice Whole cell immobilization technique (any one)
- 10. Practice Enzyme immobilization technique and its kinetics
- 11. Isolation and estimation of DNA and RNA
- 12. Isolation of plasmids and expression of protein
- 13. Agarose gel electrophoresis of DNA/ RNA
- 14. SDS – polyacrylamide gel electrophoresis for proteins.
- 15. Microbiological assay of antibiotics.
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Recommended Textbooks & References
- 1. Baltz, R. H., Davies, J. E. and Demain, A. L. Manual of Industrial Microbiology and Biotechnology. ASM Press.
- Carter, S. J. Cooper and Gunn’s Tutorial Pharmacy. CBS Publishers & Distributors.
- Chou, S. L. M. Practical Biotechnology: A Guide to Biochemical Engineering. Springer.
- Denyer, S. P., Hodges, N. A. and Gorman, S. P. Hugo and Russell’s Pharmaceutical Microbiology. Wiley-Blackwell.
- Glick, B. R., Pasternak, J. J. and Patten, C. L. Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press.
- Lachman, L., Lieberman, H. A., Kanig, J. L. and Khar, R. K. The Theory and Practice of Industrial Pharmacy. CBS Publishers & Distributors.
- Pelczar, M. J., Chan, E. C. S. and Krieg, N. R. Microbiology. McGraw-Hill.
What's coming next on this page
- Previous year question papers (PYQ)
- Topic-wise short notes and revision summaries
- MCQs and self-assessment quizzes
- Suggested external resources and video tutorials
Other subjects in Semester 4
BP401THerbal Drug TechnologyBP402TMedicinal ChemistryBP403TPharmaceutical BiotechnologyBP404TSocial Pharmacy and Public HealthBP405TSystemic Pharmacology IBP406PHerbal Drug Technology (Practical)BP407PMedicinal Chemistry (Practical)BP409PSocial Pharmacy and Public Health (Practical)BP410PSystemic Pharmacology I (Practical)BP411IInternship I (Mandatory)