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BP605TSemester 63 creditsTheory

Pharmaceutical Analysis

Complete unit-wise syllabus for BP605T as per the PCI B.Pharm NEP 2020 curriculum (Semester 6 — AI in Pharma + Analysis + Internship II).

All Sem 6 Subjects
URL:https://pharmacode.vercel.app/syllabus/semester-6/bp605t-pharmaceutical-analysis/

Unit-wise Syllabus

5 Units
1
Electroanalytical Methods9 Hours
  • Potentiometry: reference and indicator electrodes (glass electrode, calomel electrode, ion-selective electrodes); measurement of EMF; pH determination; potentiometric titrations
  • Voltammetry: polarography; direct current, AC polarography; differential pulse polarography; pharmaceutical applications
  • Conductometry: principle, conductometric titrations; applications in pharmaceutical analysis
2
Spectroscopic Methods9 Hours
  • UV-Vis spectrophotometry: Beer-Lambert law; chromophores and auxochromes; single and double beam spectrophotometers; applications — assay of vitamins, antibiotics, drug purity testing
  • IR spectroscopy: molecular vibrations (stretching, bending); functional group identification; KBr pellet method, ATR; interpretation of spectra; pharmaceutical applications
  • Fluorimetry: principle; fluorescence spectrometers; pharmaceutical applications; advantages and limitations
3
Atomic Spectroscopy and Flame Methods9 Hours
  • Atomic absorption spectroscopy (AAS): principle, instrumentation (hollow cathode lamp, flame/graphite furnace atomization), interferences; pharmaceutical applications (heavy metal testing)
  • Atomic emission spectroscopy (AES/ICP-OES): principle, plasma sources; applications in elemental analysis of pharmaceuticals
  • Flame photometry: principle, instrumentation; alkali metal determination (Na, K, Li); pharmaceutical applications
4
Chromatographic Methods9 Hours
  • HPLC: principles, column types (RP-HPLC, HILIC, ion exchange, size exclusion); detectors (UV, fluorescence, ELSD, RI); method development; pharmaceutical applications (assay, impurity profiling)
  • GC: principles; stationary phases; injection techniques (split, splitless, on-column); detectors (FID, ECD, NPD, MS); applications in residual solvents, volatile impurities, essential oil analysis
  • Ion chromatography, affinity chromatography, gel filtration: principles and pharmaceutical applications
5
Mass Spectrometry and Hyphenated Techniques9 Hours
  • Mass spectrometry: ionisation techniques (ESI, APCI, MALDI, EI); mass analysers (quadrupole, TOF, ion trap, Orbitrap); fragmentation patterns; molecular weight determination and structural elucidation
  • LC-MS and LC-MS/MS: principle; SRM/MRM quantitation; pharmaceutical applications — impurity profiling, bioavailability studies, metabolite identification
  • GC-MS: EI fragmentation; spectral libraries (NIST); quantitative analysis; applications in residual solvent analysis, forensic pharmacy, pesticide residue analysis

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